A unique Software Package for UV/VIS Spectroscopy
UV/Vis Spectroscopy is a technique that is widely used to characterize, identify and quantify chemical compounds in all fields of analytical chemistry. UV/Vis Spectroscopy plays an important role in the field of water- and environmental analysis, biotechnology, food analysis, clinical chemistry pharmaceutical analysis and many more applications.
Almost in any analytical discipline you will find applications for UV/Visible Spectroscopy - in many cases for quantification purposes.
UV/Vis Spectroscopy plays an especially impor-tant role in routine analytics due to its simple applicability and manifold application fields. Routine analytics often means the quantification of a certain compound often with a certain sample pre-treatment before the actual analytical step. Routine analysis also means that there are many samples to be analyzed on a regular base using the same analytical methodology. This often requires a calibration with a series of calibration standards and the establishment of a calibration curve.
Result calculation often requires that certain calculations are applied to the raw absorbance (or transmittance) data. Typically many samples have to be measured, by lab technicians who are often not so experienced with the specific requirements and theoretical backgrounds of UV/Vis spectroscopy. Therefore it is crucial to use a flexible software package, which easy and safe to use - despite its complexity. BLStudio is such a software package which is dedicated to calculate results from UV/Vis spectroscopy data and store them in a single file together with a customized report
Ease of use
The BioLight Studio Software package was designed for intuitive operation from the very start:
At the most direct level, this offers the researcher an easy-to-use, walk up system. Open a method, click on ‘Start’, and you’re up and running. Measure samples till you are finished, then click on stop. All data from the current sample batch is saved automatically in a single multifile: No more searching the hard disk to find which data belongs to an experiment, because all the data from the current experiment is stored conveniently in one file. Additionally all data can easily be copied and pasted into Word or Excel.
At the other extreme, BioLight Studio allows the user to add sophisticated programmable calculations, automated concentration calibrations, and detailed but easily user-designed reports. And all steps in between. And all of this can be automatically applied to all samples within a batch for high throughput, routine users.
In the basic version of BioLight Studio all records are stored in a file system. Thus it is possible to use the Windows file explorer to easily identify and archive results and methods.
Integrated Sample Handling
All sample information are defined in a customizable table structure. Specific sample information like a dilution factor can easily be added to the table. This information can be used in calculations and be inserted into reports.
When a series of samples is to be measured, the Samples page is used to define the number and type (Blank, Reference, Unknown).
Sampling accessories (for example, the Autosampler or the Peltier temperature controller) are fully integrated into all data acquisition types in the system. This means that the user can automatically sample and quantitate samples using the Autosampler.
Setting Instrument Parameters - it can't be easier
Instruments parameters are visualized showing the light-path diagram, together with the relevant instrument components.
So it is very straght-forward to define the scan wavelengths, scan speeds, the light sources, the slit settings, the cell holders and the measurment mode - Absorbance, Transmittance and other instrument settings.
These parameters are accesible in the "Acquisition" tab of the Biolight Studio Software Package.
The basic version BioLight Studio offers 3 calculation modules that allow to perform an automated data processing during the measurement: The standard calculator, the concentration calculator and the 3D viewer.
The screen shot to the right shows a series of scans, where the peak areas and the peak heights were calculated automatically. In addition the readings at certain wavelengths had been extracted. To obtain spectral purity information the ratio at certain wavelengths had been calculated. Finally, even, a decision was introduced. In the case that the calculated ratio is below a certain given threshold an information will be output, to tell the researcher that the substance is not anymore spectrally pure.
The screen shot to the left displays the concentration calculator. In this example a calibration curve based on 4 references was calculated and displayed. The calculated concentration is shown for each of the six measured samples, together with the sample ID, the measured signal, and dilution factor.
All calculators allow the user to easily copy the results, either as tabular data or as vector-graphic, to the clipboard.
From there they can be pasted directly into word processing or table calculation programs for further evaluation and reporting. Of course all results can be inserted into the BioLight Studio report module without the need for an external program.
In addition to the automatable calculation modules, a stand-alone calculator is delivered free of charge. Your colleagues can analyse and re-process raw data and results off-line, free of charge.
The BioLight Studio report system is very intuitive and easy to use, offering a comprehensive array of information which can be quickly added to the report template.
Even for the casual user, a report can be produced which precisely describes all aspects of the experiment. Quickly.
The example to the left shows a report for the measurement of Nitrate concentration in contaminated water. Six standards and three unknown samples were measured. The report includes the relevant measurement parameters and a calibration curve which displays the position of standards and samples within the curve. The raw data, together with the calculated concentrations, are displayed together with sample name and sample information.
BioLight Studio stores the report template together with the raw data, rather than the final report, thus allowing the user to add or remove results, tables and graphs and re-generate the report whenever required. The final reports can be printed or exported in various formats, including the .pdf format.
21 CFR Part 11 Support
In the basic version BL Studio identifies the current user from the Windows Login. It is possible to assign different method and data directories for each user.
All data are stored in binary records, secured with a checksum. To ensure the reproducibility of results all raw data are stored together with the acquisition methods, all calculation parameters and the report template.
On opening a Biolight Studio data set, the method used to collect the data is automatically re-loaded in its entirety. New data can be measured using exactly the same conditions as before.
All methods, results, configuration data and protocol records support electronic signatures. In the basic version electronic signatures are added automatically whenever new results or methods are stored.
To allow for the traceability of results BL Studio offers a comprehensive audit trail. The audit trail is organized as one record per day, thus allowing to easily archive data!
Instrument Performance Verification
The Instrument Performance Verification module is provided with the BL Studio, to allow the user to regularly check instrument performance. The Validation module automatically carries out a series of tests to determine the instrument’s performance in terms of wavelength accuracy, sensitivity and drift.
Data is displayed in real-time, and the results are automatically saved for later archiving. A report can be printed, or copied to the clipboard for further processing with a word processing program.
BioLight are constantly developing new, plug-in applications. These can be easily added to the system individually: The system structure remains unchanged. This means minimal re-validation, no down-time and instant expansion of the Biolight Studio.